Abstract: Objective To study the relation of insulin-induced gene 2 (INSIG2) rs7566605 with obesity and lipid metabolism in Chinese populations. Methods A total of 2014 healthy elderly subjects were sampled from Beijing in September 2009-June 2010. Their genotypes of DNA were detected. Their serum levels of triglyceride and HDL-C were measured. The relation of different rs7566605 genotypes with obesity and abnormal lipid metabolic diseases was analyzed. Results The rs7566605 genotypes were related with the increased serum triglyceride level and decreased serum HDL-C level in males (P=0.029), which might increase the risk of hypertriglyceridemia (P=0.019, OR=1.175) and low lipoproteinemia (P=0.020, OR=1.178). Heterogeneous dominant model might be the major genetic model of rs7566605. However, the rs7566605 genotypes were not related with BMI or obesity. Conclusion The rs7566605 genotypes are related with lipid metabolism but not with obesity in elderly Chinese populations.