贝伐珠单抗引起肝癌细胞HepG2的血管内皮生长因子受体2上调表达

Bevacizumab up-regulates expression of VEGFR2 in HepG2 cells

摘要: 目的探讨贝伐珠单抗对肝癌细胞HepG2血管内皮生长因子（vascular endothelial growth factor，VEGF）相关受体的影响。方法通过不同浓度的贝伐珠单抗降低培养液中VEGF来模拟低浓度VEGF的肿瘤微环境，RT-PCR、ELISA、Western blotting检测肿瘤细胞VEGF相关受体表达水平的变化。结果细胞培养液的VEGF浓度大幅度下降（P< 0.05）；可溶性VEGFR2的浓度出现了上升（P< 0.05）；HepG2细胞VEGF和VEGFR1表达水平无明显变化（P> 0.05）；VEGFR2表达水平出现了明显的上调（P< 0.05）。结论贝伐珠单抗导致了肝癌细胞HepG2大幅度上调了VEGFR2的表达。

Abstract: Objective To study the role of bevacizumab in up-regulation of VEGFR2 expression in HepG2 cells. Methods Tumor microenvironment was simulated by decreasing the VEGF concentration with bevacizumab monoantibody. Expression of VEGF2 in HepG2 cells was detected by RT-PCR, ELISA and Western blot, respectively. Results The VEGF concentration was signi fi cantly decreased (P< 0.05) and the soluble VEGFR2 concentration was signi fi cantly increased in medium (P< 0.05). No signi fi cant change occurred in the VEGF and VEGFR1 expression level (P> 0.05) whereas the expression of VEGFR2 was signi fi cantly upregulated in HepG2 cells (P< 0.05). Conclusion Bevacizumab up-regulates the expression of VEGF2 in HepG2 cells.