Abstract: Objective To study the influence of the spatial factor which is closely associated with spacemen's health, the whole genome sequence of Pseudomonas aeruginosa strain LCT-PA220. Methods The characteristic was identified by gram stain examination. Then genomic DNA was extracted from LCT-PA220 strain and the library was constructed and followed by sequencing. The raw date were fltered, assembled into genome, annotated and analyzed. Results The identify of LCT-PA220 strain was gram-negative bacteria whose genome was 6.87M containing 54 tRNA genes and 5 829 coding sequences with 962 bp average gene length. COG and KEGG annotation analysis revealed 22 COG functions and 32 KEGG pathways, respectively. Conclusion The whole genome of LCT-PA220 strain is sequenced and annotated well, which paves the way for further study of this strain.