Abstract: Objective To provide the evidence for improving the general literacy of junior nurses by investigating their demand for training in rotation and the current situation of training in rotation． Methods The current situation of training in rotation and the demand for training in rotation were investigated in 1 183 junior nurses of 14 class A hospitals and 4 class B hospitals in Beijing using the questionnaire we designed． Results Of the investigated junior nurses, 10．5% considered that the training in rotation should begin in related departments and large departments with intravenous transfusion puncture as their main training content after they worked for 1 year, 37．3% demanded that the training in rotation should begin in related departments, emergency department and operating room with intravenous transfusion puncture and frst-aid as their main training contents after they worked for 1 year． The junior nurses with a record of secondary specialized schooling held that they should be trained in large departments and operating room in rotation (P< 0．05), those with a record of higher specialized schooling believed that they should be trained in emergency department, ICU and electrocardiogram department in rotation (P< 0．05), those with a record of regular college or above schooling thought that they should be trained in related departments in rotation (P< 0．05)． The demand for training in training bases was greater in junior nurses with a record of secondary specialized schooling than in those with a record of regular college or above schooling (P< 0．05)． Conclusion Training in rotation can cultivate nurses with multiple subject backgrounds by improving their know ledge, skill and professional level．