Abstract: The presence of micro-gravity space environment, high vacuum, extreme temperature, weak magnetic field and particle radiation and other factors can induce proteomics changes in bacteria, thereby affecting the biological characteristics and functions of microorganisms. Studies find that proteomics of Serratia marcescens, Bacillus cereus, Salmonella typhimurium, Escherichia coli and Enterococcus faecium will change when induced by the space environment. The recent progress in the researches on proteomics of bacteria affected by space environment is reviewed in present paper in the hope of providing novel way for analysis of bacterial activity in space environment.