Abstract: Objective To investigate the correlation of intracranial pressure (ICP) and intraocular pressure (IOP) changes in patients with optic nerve disease. Methods Clinical data about 12 patients with optic nerve disease admitted to our hospital from January to May in 2013 were retrospectively analyzed. The quantitative cerebrospinal fluid of all patients during hospitalization were extracted by lumbar puncture, and after that routine inspection of cerebrospinal fluid, the measurement of ICP were performed. The changes of before and after lumbar puncture were measured using spring back tonometer and the average value were calculated. 24-hour intraocular pressure curve was described with all patients undergoing the lumbar puncture once every 2 hours. Results Of the 12 cases (24 eyes), 7 cases were male and 5 cases were female with the average age of (38.125 ± 12.438) years old (range, 16-55 years old). 6 cases were diagnosed as optic neuritis, 5 cases were ischemic optic neuropathy, and 1 case was undefined diagnosis. All patients were in good condition with no high blood pressure, diabetes or any other common systemic disease. There was no significant correlation between before and after lumbar puncture ICP and eyes mean IOP (r=0.126, P=0.697). The differences of before and after lumbar puncture in IOP (t=8.002, P＜ 0.001) and ICP (t=7.936, P＜ 0.001) were statistically significant. Conclusion There is a certain correlation between IOP and ICP, but no regularity is found between the changes of them. Meaningful conclusion about presuming the IOP basing on the ICP or presuming the ICP basing on the IOP is yet to be educed.