Abstract: With the expanded and deepened of basic research fields, the refractory of gastric cancer is generally attributed to the existence of gastric cancer stem cells and the abnormal expression of signal pathways, among which Wnt/β-catenin signal pathway is the most representative. As the receptor protein and target gene of Wnt/β-catenin signal, Lgr5 is also considered as the potential biomarker of many malignant tumor stem cells. The relationship between Lgr5-Wnt/β-catenin signal pathway and gastric cancer stem cells is reviewed combining with the current research progress.