Abstract: Objective To investigate the relationship between uric acid (UA), high sensitivity C reactive protein (hs-CRP) and ambulatory arterial stiffness index (AASI) in patients with non-dipper hypertension. Methods One hundred and seventy-six patients admitted to our hospital from January 2013 to January 2014 were divided into dipper hypertension group (n=81) and non-dipper hypertension group (n=95) and other 76 patients of normal blood pressure served as the control group. All groups were tested UA and hs-CRP and given 24 h ambulatory blood pressure monitoring. The relationship between UA, hs-CRP and AASI were analyzed. Results Compared with dipper hypertension group and the control group, non-dipper hypertension group had long pathogenesis, high systolic blood pressure, low diastolic blood pressure and big pulse pressure (P< 0.05). UA, hs-CRP and AASI of non-dipper hypertension group were significantly higher than others (P< 0.05), and UA was a risk factor for non-dipper hypertension. UA (r=0.436, P< 0.001) and hs-CRP (r=0.603, P< 0.001) were positively correlated with AASI. Conclusion With the increase of hs-CRP and UA, AASI becomes higher and has more serious degree of arteriosclerosis in patients with non dipper hypertension.