Abstract: Pancreatic cancer is a kind of malignant tumor which is very difficult to diagnose and cure. Researches in developing convenient serological markers in screening the early curable stage of pancreatic cancer have important clinical significance. At present, the study of serological marker of pancreatic cancer includes the molecular of pancreatic cancer-specific expression, gene mutations, abnormal methylation of gene DNA, microRNA and circulating tumor cells. Combinations of different serological markers can contribute to the early diagnosis of pancreatic cancer, and further researches should focus on the screening of high-risk groups with pancreatic cancer and the development of highly sensitive and specific markers, in order to improve the diagnostic level of pancreatic cancer.