Abstract: The detection of bacterial drug resistance is essential for guiding the treatment of many types of bacterial infections. The most commonly employed method is antimicrobial susceptibility testing. This method requires that the pathogens are isolated from the clinical samples before testing, and it is complicated and also takes time. In recent years, some molecular biology techniques have made great progress in the detection of bacterial drug resistance, but still need to be improved in clinical practice. This paper aims to highlight several techniques and provide references for rapid pathogen resistance detecting in clinical practice.