Abstract: Objective To explore the relationship between quantitative analysis in thyroid nodules detected by real-time contrastenhanced ultrasound (CEUS) and characteristics of benign or malignant thyroid nodules. Methods Dynamic images detected by contrast-enhanced ultrasonography of 93 thyroid nodules from 79 patients who underwent surgery and contrast-enhanced ultrasonography before treatment in Chinese PLA General Hospital from April to August in 2014 were analyzed. The characteristics of enhancement and perfusion of thyroid nodules were observed. The filling intensity of both periphery and interior of the nodule was analyzed by time-intensity curve (TIC). The surrounding thyroid tissue region of interest (ROI) was used as reference. The relative evaluated parameters of interior of the nodule within ROI, including relative peak time (R_TTP), relative peak intensity (R_PI), relative mean transit time (R_MTT) and relative area under the curve (R_AUC), were observed. Results The R_PI, R_TTP, R_MTTin malignant thyroid nodules were less than benign thyroid nodules, which showed statistical difference (P< 0.05). R_PIand R_AUCin thyroid nodular goiter were less than thyroid adenoma, which also showed statistical difference (P< 0.05). Conclusion Quantitative analysis of characteristics of thyroid nodule detected by real-time contrast-enhanced ultrasound has certain clinical significance in differentiation of benign and malignant thyroid nodules. The nodules that are fast forward quickly out and show low enhancement in angiography should be paid attention to check out whether it is malignant. Ultrasound guided puncturing is recommended to make a clear diagnosis.