Abstract: Objective To explore the effects of multiple electrolytes injection as cardiopulmonary bypass (CPB) priming solution for patients with hepatic dysfunction undergoing open heart surgery. Methods Clinical data about 41 patients with hepatic dysfunction who underwent cardiac surgeries with CPB in our hospital from July 2003 to December 2013 were retrospectively analyzed. Lactate Ringer's solution was used as CPB priming fluid before October 2009 and then multiple electrolytes injection were used as priming fluid. They were divided into two groups:lactate ringer's group (group A, n=18) and multiple electrolytes injection group (group B, n=23). BE, Lac and electrolytes levels were detected at pre-CPB (T1), 30 minutes after CPB (T2), off-CPB (T3), postoperative 4 hours (T4) and 24 hours (T5), hepatic enzymes of AST, GPT and blood glucose were measured at preoperation, postoperative 4 hours, days 1, 3, and 7. Results The levels of AST and GPT increased notably after CPB in two groups, but it returned to baseline on postoperative 7 d in group B; the concentrations of Lac, BE, Cl- were significantly higher in group A than those of group B (P< 0.05), the plasma calcium level was lower than that of preoperation in group B. There was no difference in blood glucose between two groups. Conclusion Multiple electrolytes injection as CPB priming solution can decrease lactate levels and alleviate the harm to liver caused by CPB, and maintain acid-base balance effectively.