Abstract: Objective To analyze the efficacy of intraoperative MRI technique in treatment of patients with deep-seated gliomas. Methods Four hundred and fifty cases with deep-seated gliomas in Chinese PLA General Hospital from January 2005 to December 2014 were collected and retrospectively studied to compare the total resection rate （TRR） between conventional microsurgery combined with functional neural navigation group and intraoperative MRI group. Results Preferential options of surgical technique were found among the various locations of deep-seated gliomas. There was no difference in age， gender and pathological grade between the two groups. TRR of intraoperative MRI group was higher in the region of thalamus-basal ganglia （75.6%）， callosum（83.8%） and surroundings of ventricle （76%） than those of conventional groups （71.0%， 76.9%， 50.0%）， no statistically significant differences were found except for ventricle and surrounding regions. Also， TRR was of no statistically significant difference between the two groups neither in high grade gliomas nor in low grade gliomas. Conclusion Intraoperative MRI achieves higher TRR in the region of surroundings of ventricle.