Abstract: Objective To declare whether silencing of miRNA153 via its anti-sense nuclear (inhibitor) induces the radio-sensitization of lung cancer cells. Methods The anti-sense nuclear acid (inhibitor) of miRNA153 was transfected into lung cancer cells, which were irradiated by 60Co-γ. The CCK-8 analysis, soft-agar and Transwell assays were performed to identify the effect of miRNA153 silencing on radio-sensitization in lung cancer cells. RT-PCR and Western blot assays were used to detect the effect of anti-sense nuclear acid (inhibitor) of miRNA153 on miRNA153, its target protein PTEN and the expression of Survivin cell proliferation of survival regulators. Results CCK-8 analysis revealed that the irradiation of medium dose ray (4 Gy) could kill the lung cancer cells (A549, H460, H1299 and H358), and the down-regulation of expression of miRNA153 could enhance the radio-sensitization in lung cancer cells. The soft-agar and Transwell assays proved that the up-regulation of rays could inhibit the anchorage-independent growth and invasion of A549 cells. And the molecular mechanism experiment indicated that the anti-sense nuclear acid of miRNA153 significantly disrupted the endogenous expression of miRNA153 and Survivin, and in turn upregulated the expression of PTEN. Conclusion Silencing of miR-153 significantly enhances the sensitivity of lung cancers.