Abstract: Objective To develop a new method to establish renal insufficiency model for cardiorenal syndrome research. Methods Twenty beagles were randomly divided into model group and sham group. In model group, embolization of some branches of renal artery was achieved by injecting gelatin sponge granules, thus leading to the establishment of renal insufficiency model. The sham group underwent the same procedure, except for embolization. After 3 months, parameters about renal insufficiency including creatinine, urea nitrogen, uric acid, lactate dehydrogenase were tested. The blood pressure and atrial fibrillation (AF) vulnerability were evaluated. The activity of the sympathetic nervous system and renal-angiotensin-aldosterone system were measured. Results After 3 months, the renal function was impaired seriously in model group for the ischemia of glomerular. Compared with sham group and baseline data, the creatinine, urea nitrogen, uric acid, lactic dehydrogenase in model group increased significantly. The systolic pressure elevated from (170.9±12.8) mmHg to (191.1±17.8) mmHg with statistically significant differences (P＜0.05). Diastolic pressure elevated from (140.4±10.9) mmHg to (126.3±19.8) mmHg with statistically significant differences (P＜0.05). There were no significant differences in related indexes in sham group before and after operation. After 3 months, the AF inducibility of model group were 41%, which was higher than baseline (25%) and sham group (22%) with significant difference (P＜0.05). The plasma levels of norepinephrine, renin, aldosterone and the angiotensinⅡlevel in atrial tissue increased significantly. Conclusion The embolization of renal artery with gelatin sponge granules can steadily establish the model of renal insufficiency, which can provide a new animal model for cardiorenal syndrome research.