Abstract: Objective To detect the overexpression of B-cell translocation gene 1 (BTG1) in BGC823 and its effect on cell cycle, apoptosis and migration. Methods Recombinant plasmid was transfected into pcDNA3.1-BTG1 cells by Liposome, then the cells were selected and divided into BTG1 plasmid group, empty vector group and blank control group. The expression of BTG1 protein in BGC823 cells was detected by western-blot. The effect of BTG1 overexpression in BGC823 on cell cycle, apoptosis and migration was detected by flow cytometry and wound healing. Results The expression of BTG1 protein was significantly higher in BTG1 group than empty vector group and blank control group (P＜ 0.05). Flow cytometry showed that G2 / M phase cells in BTG1 group was significantly higher than other groups (21.38%, 18.47% vs 8.57%, 8.74%, P＜ 0.05), while early apoptosis and total apoptosis rate increased significantly (3.41%, 3.05% vs 1.32%, 0.87% and 6.12%, 8.30% vs 3.17%, 2.34%, P＜ 0.05). Conclusion High expression of BTG1 can induce the retardation of G2/M, inhibit cell proliferation and increase the rate of apoptosis.