Abstract: Objective To analyze the source and constitution of pathogens causing ocular infection in elderly patientsduring perioperative period of cataract surgery and explore its relative preventive measures. Methods In this retrospective study conducted in Chinese PLA General Hospital, clinicaldata about 80 eyes (80 patients aged ≥ 60 years) were collected from March 2014 to February 2015 and analyzed by SPSS 20.0 statistical software. Results The positive rate of bacteria culture was significantly higher in palpebral magin (65%) than conjunctival sac (37.5%), while there were tinydifferences in the components of pathogens in two sites with G⁺coccus as the most common seen bacteria; after the use of 0.5% levofloxacin eyedrops, the positive rate of bacteria culture in palpebral magin and conjunctival sacdecreased significantly (40%/20%). With the application of PVP-iodine solution, surgical membrane and tobramycin solution, the bacteria cultu