Abstract: Objective To investigate the influence ofdifferent burndepth in skin of rats on early albumin exudation. Methods With the help of metal head of constant temperature welding machine, either side of the abdomen was heated in 16 adult male SD rats at 78℃ respectively which caused skin moderate anddeep burns. 0.5% Evans blue (1 ml/250 g) was injected from tail vein at 5 minutes after burned. Samples were taken indifferentdepth of burn and non-burned areas 3 h later and each sample was placed in formamide and incubated for 24 h in a shaking water bath at 50℃ to extract Evans blue from the tissue and the OD values of formamide samples were measured. The Evans blue extravasation in rat's skin represented the amount of albumin exudation. Results Formamide solution ofdifferent concentrations of Evans blue and its OD value showed a highdegree of correlation. The content of Evans blue of nonburned, moderate burn anddeep burn skin tissues were (29.4±6.4) μg/g, (146.4±28.7) μg/g, (308.6±25.2) μg/g, respectively. The amount of albumin exudation in the rat's skin of moderate burn anddeep burn increased about 5 and 10 times, respectively. Conclusion In the early stage of burn injury, a large amount of albumin loses in vitro, and the loss of albumin increases with the increase of thedepth of burn.