Abstract: Objective To analyze the predictive value of combined detection of procalcitonin (PCT), C-reactive protein (CRP) and interleukin-6 (IL-6) for prognosis of sepsis. Methods One hundred and sixty-two patients with spesis were collected retrospectively from January 2005 to January 2015 in Chinese PLA General Hospital.All patients were divided into survival group (n=83) and death group (n=79) according to the outcome at 28 days after admission.The differences of PCT, CRP, IL-6, SOFA score, APACHEⅡscore and MEDS score were compared between two groups.The accuracy of PCT, CRP and IL-6 in prediction of prognosis of sepsis was also evaluated. Results Of the 162 patients, there were 102 males (63.0%) and 60 females (37.0%) with age ranging from 19 to 83 years average age of (57.06±15.37) years old.The number of patients with angiocardiopathy, central nervous diseases, digestive diseases, PCT, IL-6, CRP, SOFA score, APACHEⅡ score and MEDS score in death group were significantly higher than survival group (P< 0.05).The area under ROC curve (AUC) for the prognosis of sepsis was 0.955 when PCT, CRP and IL-6 were detected in combination, which was higher than the AUC of single detection of PCT (AUC=0.941), CRP (AUC=0.780) and IL-6(AUC=0.884).Sensitivity (86.5%), specificity (96.4%), positive predictive value (+PV, 95.6%) and negative predictive value (-PV, 85.1%) of combined detection were higher than single detection of PCT (sensitivity 77.2%, specificity 95.2%, +PV 93.8%, -PV 81.4%), CRP (sensitivity 72.2%, specificity 80.2%, +PV 75.8%, -PV 75.8%) and IL-6 (sensitivity 86.1%, specificity 72.3%, +PV 74.7%, -PV 84.5%). Conclusion PCT, CRP, IL-6 are important factors for predicting the prognosis of sepsis.The combined detection of PCT, CRP and IL-6 is more effective than single detection in estimating prognosis of sepsis.