Abstract: Objective To investigate the value of magnetic resonance imaging combined with intraoperative ultrasound in resection of deep-seated brain tumors. Methods A retrospective analysis in 49 patients who underwent deep-seated brain tumors resection by magnetic resonance imaging alone or combined with intraoperative ultrasound in our hospital from July 2014 to July 2015 was carried out.Of the 49 cases, 22 cases were treated with intraoperative magnetic resonance imaging assisted tumor resection, 27 cases with intraoperative magnetic resonance imaging combined intraoperative ultrasound assisted tumor resection.The tumor resection extent, KPS score, surgical time, scanning times and brain shift were analyzed. Results There were no statistically significant differences in age, gender, tumor location, tumor grade, preoperative KPS score between two groups (P> 0.05).The tumor resection extent, KPS score, surgical time and scanning times in research group were (92.01%±6.17%), (76.15 ±10.36), (2.9±0.6) h, (1.29±0.43), respectively, which were all better than intraoperative magnetic resonance imaging group, while no significant differences were found in tumor resection extent and KPS scores between two groups.There were 3 cases with brain shift after dural incision, and they were corrected under ultrasound with satisfactory results. Conclusion Application of intraoperative ultrasound can reduce the scanning times of magnetic resonance imaging, shorten surgical time, correct the brain shift in time and complete the tumor resection effectively.