Abstract: Objective To study the effect of overexpressed Trefoil factor family 2 (TFF2) on proliferation of human gastric carcinoma cell line AGS. Methods The recombinant plasmid pcDNA3.1-Tff2, including TFF2 cDNA, was transfected into AGS cells.Western blot was used to detect the expression of TFF2 protein.The effects of TFF2 on proliferation and apoptosis of AGS cells were analyzed using CCK-8 and Annexin V/PI. Results After successfully transfection of pcDNA3.1-Tff2, the expression of TFF2 was 5 times higher in protein level than that in empty plasmid group and control goup, and the proliferation of AGS cells in pcDNA3.1-Tff2 transfection group was inhibited significantly.Compared with empty plasmid group, CCK-8 assay showed that 96h OD490nm absorbance in pcDNA3.1-Tff2 group decreased significantly (0.296 vs 2.108, P< 0.01), while the percentage of cell apoptosis in pcDNA3.1-TFF2 group elevated significantly (15.1%±1.21%) vs (5.49%±0.32%), P< 0.01. Conclusion The overexpression of TFF2 can significantly decrease cell proliferation and promote apoptosis of AGS cell line, which may be an effective method in inhibiting the progress of gastric cancer.