Abstract: Objective To explore the effects of intragastric administration of alcohol on anesthesia effect and antioxidant activity in mice with propofol anesthesia. Methods Forty mice were divided into intragastric administration of saline group (NS group), intragastric administration of saline and propofol 80 mg/kg group (PPF group), intragastric administration of 50% absolute ethanol 6 ml/kg and intraperitoneal injection with saline group (Alcohol group), intragastric administration of 50% absolute ethanol 6 ml/kg and propofol 80 mg/kg group (Alcohol+ PPF group) with 10 in each group.The mice were anaesthetized by intraperitoneal injection with saline or propofol at 10 minutes after administration of alcohol or 0.9% saline.The anesthesia induction and maintenance were observed and recorded.The SOD and MDA in serum of all groups were measured after righting reflex recovered. Results Compared with PPF group, the induction time of Alcohol+ PPF group was shorter and maintenance phase of Alcohol+ PPF group was longer with significant differences (3.19±0.48) min vs (1.43±0.19) min, (67.01±6.46) min vs (183.41±12.60) min, P< 0.01; Compared with NS group, the SOD level in serum of Alcohol group, PPF group and Alcohol+ PPF group decreased significantly (38.50±6.02) U/ml vs (33.09±3.81) U/ml, (33.91±4.62) U/ml, (30.98±3.51)U/ml, P< 0.01; Compared with NS group, the MDA level in serum of Alcohol group, PPF group and Alcohol+ PPF group increased significantly (5.21±0.92) nmol/ml vs (9.16±1.85) nmol/ml, (9.37±1.52) nmol/ml, (10.14±1.76) nmol/ml, P< 0.05. Conclusion Alcohol can reduce anesthesia induction time, prolong maintenance phase, decrease the activity of serum SOD and increase the level of MDA in mice with propofol, which has important clinical significance to anesthetic selection for alcoholics.