Abstract: Objective To discuss the efficacy of intestinal micro-ecological preparations (trigeminy viable organism powder) in adjunctive therapy to patients with severe acute pancreatitis. Methods Sixty-eight patients with severe acute pancreatitis who were treated in our hospital from January 2013 to December 2015 were selected, and they were randomly divided into experimental group (n=34) and control group (n=34).In addition to the basic treatment of fasting, inhibition ofgastric and pancreatic secretion, restoring water and electrolytes balance, nutritional support, anti-infection and so on, patients in experimental group were further treated with micro ecological preparation- Bifico.Then the inflammatory indicators, incidence of complications of patients in each group after treatment were compared. Results There were no significant differences in patients'gender, age, APACHE-Ⅱ score at admission, Ranson score, organ dysfunction, etc (P＞ 0.05).After treatment for 14 days, the inflammatory indicators in experimental group were significantly lower than control group CRP: (53.1±16.9) mg/L vs (62.9±19.7) mg/L, WBC: (7.1±1.6)×10⁹/L vs (8.2±1.9)×10⁹/L, AMY: (265.8±46.2) U/L vs (291.6±51.8) U/L, ET: (0.12±0.03) EU/ml vs (0.15±0.04) EU/ml, LDH: (45.8±11.2) U/L vs (52.1±13.7) U/L, P＜ 0.05, and the patients’ recovery time ofgastrointestinal function, incidence of pancreatic infection, hospitalization time in experimental group were also significantly lower than control group (8.2±2.7) d vs (9.8±3.1) d, 14.7% vs 38.2%, (19.5±3.4) d vs (21.1±2.9) d, P＜ 0.05.Meanwhile, there was no significant difference in mortality between two groups (5.9% vs 11.8%). Conclusion Based on the comprehensive treatment, intestinal micro-ecological preparations is important in the adjuvant treatment in alleviating the inflammatory response, reducing the infection rate and promoting the recovery ofgastrointestinal function in patients with SAP.