Abstract: Objective To investigate expression changes of albumin D site-binding protein (DBP)gene in the anti Thy-1 nephritis model and prove that clockgenes are involved in the regulation of the pathogenesis of mesangial proliferativeglomerulonephritis (MsPGN). Methods MsPGN model was established by tail intravenous injection of anti-Thy-1 antibodies in SD rats, and the animals were scarified at 10 am and 4 pm on day 3 and day 5 after the injection.Animals in normal control group were injected with PBS and scarified at 10 am and 4 pm on day 0.Five rats were used at each time point.PAS staining was used to examine mesangial proliferation and extracellular matrix accumulation.Total RNA was extracted from kidney tissue by Trizol method, then qRT-PCR was used to detect the DBPgene expression. Results On day 1 after the injection, mesangiolysis appeared with inflammatory cell infiltration.On day 5, mesangiolysis disappeared and mesangial cells began to proliferate with extracellular matrix accumulating significantly.DBP mRNA level inglomeruli was higher at 10 am on day 3 and day 5, and it was also higher at 4 pm on day 5 compared to control group.However, there was no significant difference in DBP expression in cortex between Thy-1 model group and control group. Conclusion The upregulation of DBP in theglomeruli of anti Thy-1 nephritis model implies that the clockgenes are involved in the progression of renal disease.