Abstract: Objective To investigate the distribution and drug resistance of carbapenem-resistant psedomonas aeruginosa (PAE) and acinetobacter baumannii (ABA) in our hospital from January 2014 to August 2015 and promote clinical rational use of antibiotic. Methods The distribution and drug resistance of the carbapenem-resistant acinetobacter baumannii (CR-ABA) and carbapenemresistant psedomonas aeruginosa (CR-PAE) isolated from clinical samples in our hospital from 2014 to 2015 were retrospectively analyzed. Results From 2014 to 2015, 156 and 517 strains of CR-PAE and CR-ABA were separated, and more than 50.0% samples were from phlegm. And 34.0% of CR-PAE and 15.5% of CR-ABA were isolated from department of hyperbaric oxygen, followed by respiratory medicine and ICU. The drug resistance became more serious as patients' age growing. Only resistance of CR-PAE to amikacin was under 30%; Except resistance of CR-ABA to Amikacin was 21.3%, resistances to other antibiotics were higher than 90.0%, and the resistances to meropenem and imipenem were 60.7% and 90.1%, respectively. Compared with PAE and ABA, resistance of CR-PAE and CR-ABA to imipenem and meropenem were significant different (P< 0.05). Conclusion Resistance of CR-PAE and CR-ABA has increased, especially in multi-drug resistance and pan-resistant strains.