Abstract: Objective To screen bladder cancer related genes and analyze the correlation between the related genes and clinical features of patients with bladder cancer. Methods The gene expression in bladder cancer tissue and adjacent normal tissue were detected using gene chip assay. The genes with different expression in cancer tissue compared with adjacent tissue were selected and verified in 34 bladder cancer patients using q-PCR. The correlation of these genes with clinical features of bladder cancer patients were analyzed. Results Total of 6 526 genes were found to be differently expressed in cancer tissue compared with adjacent tissue by gene chip assay. Twelve genes with greater differences were chosen for further identification in 34 samples. The expressions of four genes (CEACAM5, MYH11, CNN1 and LY6D) were found to be consistent with the results of gene chip assay. In the further investigation, we found that the levels of CEACAM5, MYH11 and CNN1 were significantly different between MIBC (muscle invasive bladder cancer) and NMIBC (non-muscle invasive bladder cancer). However, the level of LY6D in MIBC didn't differ with NMIBC. Meanwhile, the correlation of genes with other clinical features was not observed. Conclusion We reveals four genes related with bladder cancer in this study, and three of them (CEACAM5, MYH11 and CNN1) may involved in tumor invasion.