Abstract: The free radical scavenger perfusion fluids includ L-arginine, ferulic acid (sodium), enzyme free radical scavenger, low molecular free radical scavenger, melatonin, traditional Chinese medicine with its extract, and edaravone etc. Different perfusion fluids will protect the ischemia-reperfusion injury of amputated limb in different degrees. Besides, whether better perfusion combination scheme, or the joint application of physical preservation of continuous close negative pressure suction will be benificial or not need to be further explored.