Abstract: Objective To explore the feasibility and short term outcome of No.6 lymph nodes dissection for gastrectomy via laparoscopic versus laparotomic approach. Methods Three hundred and eighty patients who underwent gastrectomy in Chinese PLA General Hospital from January 2014 to December 2014 were identified. Ninety-seven of them were treated by laparoscopic resection, and the rest of the cases underwent laparotomy. The perioperative condition and follow-up data of two groups were retrospectively compared. Results No significant difference was found in age, gender, postoperative pathologic staging results, the number of dissected No.6 lymph nodes between two groups (P> 0.05). However, laparotomy was superior to laparoscopic resection in operating time(18.72±3.62) min vs (25.85±2.26) min, P < 0.001. Meanwhile, local recurrence was not observed during the short-term follow-up of 6-18 months. Conclusion No.6 lymph nodes dissection in laparoscopic gastrectomy is safe and feasible when operated by experienced surgeons.