Abstract: Objective To identify the expressions of miR-144-3p and transmembrane protein 16 (TMEM16A) in osteosarcoma 143B cells and explore the effect of miR-144-3p which may regulate TMEM16A expression on cell proliferation and invasion. Methods MiR-144-3p was predicted by bioinformatics which may regulate TMEM16A expression. After transfection of miR-144-3p mimics or siRNA against TMEM16A into cells, expressions of miR-144-3p and TMEM16A were detected by real-time PCR or western blot. The proliferation and invasion of 143B cells were respectively determined using MTT assay and transwell chamber assay. Results MiRanda and TargetScan showed that there was a good complementary relationship between miR-144-3p and TMEM16A gene. Results of real-time PCR and western blot showed over-expression of miR-144-3p downregulated the expression levels of TMEM16A mRNA and protein. The proliferation and invasion of 143B cells were suppressed after transfection of miR-144-3p mimics or siRNA against TMEM16A. Conclusion MiR-144-3p may inhibit TMEM16A expression in osteosarcoma 143B cells and subsequently suppress cell proliferation and invasion.