Abstract: Objective To investigate the influence of intracranial cryptococcal infection on brain barrier permeability. Methods Twelve adult male SD rats weighting 260-300 g were randomly divided into control group and experimental group (n=6 in each group).Cryptococcal meningitis rat model was established in experimental group.Fluconazole was administered via the tail vein and micro dialysis sampling was used to obtain extracellular fluid and cerebrospinal fluid synchronously.The concentrations of fiuconazole in plasma, extracellular fiuid and cerebrospinal fiuid were measured using high performance liquid chromatography (HPLC).The permeation rate of fluconazole in blood brain barrier and blood cerebrospinal fluid barrier in different groups were calculated to investigate the in fiuence of intracranial cryptococcal infection on brain barrier permeability. Results The permeation rates of fiuconazole through blood - brain barrier and blood - cerebrospinal fluid barrier were 62.12%±10.38% and 69.33%±7.34% respectively in the normal group, signi ficantly higher than those of 78.18%±2.45% and 87.29%±7.11% in the experimental group (P＜ 0.05, respectively). Conclusion Intracranial cryptococcal infection can increase the permeability of blood - brain barrier and blood - brain fluid barrier.