Abstract: Objective To evaluate the sealing potential of a new surgical system which applies low temperature plasma (NTS-100) in mesenteric vascular, gastric vein, splenic vein and abdominal wall vein． Methods Twelve piglets were randomly divided into three groups, and each group underwent operation using NTS-100, Valley or PEAK surgical systems, respectively． The effectiveness of sealing in the mesenteric vascular, the gastric vein, the splenic vein and the abdominal wall vein was evaluated and compared． Results Successful sealing rate of NTS-100 on the mesenteric vascular with average diameter of 1．5 mm was 100%, and the delayed bleeding rate was 0． No statistically significant difference was found when compared with Valley and PEAK (P> 0．05); Successful sealing rate of NTS-100 on the gastric vein, splenic vein and abdominal wall vein with average diameter of 3．56 mm was 100%, and the delayed bleeding rate was 0． Vessels with the greatest diameter of 4．53 mm could be sealed successfully． While, there was no statistically significant difference when compared with Valley and PEAK (P> 0．05)． Conclusion A new surgical system which applies low temperature plasma (NTS-100) has favorable effectiveness on sealing vascular when compared with Valley and PEAK in the operations on the mesenteric vascular, the gastric vein, the splenic vein and the abdominal wall vein．