Abstract: Objective The miniature pig was used to establish the animal model of seawater immersion wound for further study of physiological and pathological changes in seawater immersion wound. Methods Ten 4-month Bama pigs and another 10 4-month GuizhouⅢ miniature pigs were soaked into seawater for 3 hours to simulate seawater immersion. Then hemodynamic and arterial blood gas parameters changes were recorded and compared between different time points and different genders. Results The heart rate (HR) and mean arterial pressure (MAP) of both Guizhou and Bama miniature pigs increased significantly HR: (100.80±8.73)/min vs (107.61±7.81)/min, P=0.033 in Guizhou group; (103.74±7.51)/min vs (111.45±4.90)/min, P=0.021 in Bama group; MAP: (108.80±12.97) mmHg vs (120.52±9.66) mmHg (1 mmHg=0.133 kPa), P=0.039 in Guizhou group; (94.76±11.25) mmHg vs(104.91±11.32) mmHg, P=0.018 in Bama group, while the O₂ partial pressure (PO₂) decreased significantly (93.45±8.26) mmHg vs(90.07±6.83) mmHg, P=0.041 in Guizhou group; (94.23±5.77) mmHg vs (91.34±7.18) mmHg, P=0.037 in Bama group. After soaking in the seawater, the HR, MAP and PO₂ of female miniature pig were significantly higher than those of male miniature pigsHR: (117.90±7.69)/min vs (93.45±6.71)/min, P=0.025 in Guizhou group; (124.5±4.93)/min vs (97.63±5.70)/min, P=0.018 in Bama group; MAP: (121.50±15.66) mmHg vs (96.83±10.96) mmHg, P=0.011 in Guizhou group, (117.65±13.34) mmHg vs(83.51±10.25) mmHg, P=0.017 in Bama group; PO₂: (92.97±7.83) mmHg vs (89.47±8.19) mmHg, P=0.036 in Guizhou group; (93.34±7.88) mmHg vs (90.23±6.77) mmHg, P=0.031 in Bama group. Conclusion We have established miniature pig model of seawater immersion wound, and got some hemodynamic and arterial blood gas indexes, which may provide basis for further medical biology research.