Relationship between ERCC1 expression of circulating tumor cell and sensitivity to platinum in patients with ovarian cancer
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摘要: 目的 研究上皮性卵巢癌（epithelial ovarian cancer，EOC）患者外周血循环肿瘤细胞（circulating tumor cell，CTC）中切除修复交叉互补基因1（excision repair cross-complementation group 1，ERCC1）表达与铂类药物敏感性的关系。 方法 以2015年3月-2017年6月本院诊治的60例EOC患者为研究对象，收集手术后化疗前及3个疗程化疗（卡铂/紫杉醇）后1周内患者外周血，利用纳米滤膜结合RNA原位杂交技术分离检测CTC及ERCC1+CTC，分析ERCC1+CTC与临床特征、铂类药物敏感性的关系。 结果 60例患者中83.3%（50例）检测出CTC，60%（36例）检测出ERCC1+CTC。将36例ERCC1+CTC患者按照化疗前后ERCC1+CTC数目变化分为化疗后ERCC1+CTC数目升高组（ERCC1+CTC-H组，12例）和ERCC1+CTC数目未升高组（ERCC1+CTC-L组，24例），两组年龄、家族史、病理类型、腹水情况、腹水细胞学情况、残余病灶、FIGO分期等临床特征无统计学差异（P均> 0.05），ERCC1+CTC-L组中62.5%（15例）铂类药物敏感，ERCC1+CTC-H组中16.7%（2例）铂类药物敏感，两组差异有统计学意义（P< 0.05）。 结论 外周血循环肿瘤细胞ERCC1阳性可预测铂类药物敏感性。
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关键词:
- 切除修复交叉互补基因1 /
- 循环肿瘤细胞 /
- 卵巢癌 /
- 铂类敏感性
Abstract: Objective To study the relationship between the expression of excision repair cross complementation group 1 (ERCC1)in circulating tumor cell (CTC) in peripheral blood of patients with epithelial ovarian cancer (EOC) and the sensitivity to platinum. Methods A total of 60 EOC patients in Chinese PLA General Hospital from March 2015 to June 2016 were enrolled. Peripheral blood of patients was collected before postoperative chemotherapy and within a week after the three courses of chemotherapy(carboplatin/paclitaxel). The Nanofiltration membrane and RNA in situ hybridization technique was used to separate and detect the CTC and ERCC1-positive CTC. The relationship between ERCC1-positive CTC and the sensitivity to platinum was analyzed. Results Of the 60 cases, CTC was observed in 50 cases (83.3%) and ERCC1-positive CTC in 36 cases (60%). According to the quantitative change of ERCC1 + CTC before and after chemotherapy, the 36 patients were divided into elevated ERCC1 + CTC group (ERCC1 + CTC-H group, 12 cases) and non-elevated ERCC1 + CTC group (ERCC1 + CTC-L group, 24 cases). No significant difference was between the two groups in age, family history, histology, ascites, ascites cytology, residual tumor, FIGO stage (P> 0.05). There were 15 cases (62.5%) in ERCC1 + CTC-L group and 2 cases (16.7%) in ERCC1 + CTC-H group were sensitive to platinum, with statistically significant difference between the two groups (P< 0.05). Conclusion The ERCC1 expression of CTC can predict the sensitivity to platinum. -
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