Abstract: Objective To screen childbearing women for TORCH infection in Chengdu over the past two years so as to provide evidence for the healthcare of childbearing women. Methods Serum TORCH-IgG and TORCH-IgM were detected by chemiluminescence immunoassay in 40 986 childbearing women in Chengdu Women and Children's Hospital from July 2015 to July 2017. Results The positive rates of TOX, RV, CMV and HSVⅠ/Ⅱ were 1.02%, 1.10%, 0.40% and 9.50% respectively in TORCH-IgM detection. The highest seropositive rate was 9.50% of HSVⅠ/Ⅱ-IgM while CMV-IgM had the lowest seropositive rate of 0.40%. Meanwhile, the positive rates of TOX, RV, CMV and HSVⅠ/Ⅱwere 5.28%, 79.03%, 96.00% and 83.96%respectively in TORCH-IgG detection. The highest seropositive rate was 96% of CMV-IgG, while TOX-IgG had the lowest seropositive rate of 5.28%. The seropositive rate of HSVⅠ/Ⅱ-IgM in the young age group (18-35 years) was significantly higher than that in the elderly age group (≥ 35 years) (9.58% vs 8.43%, P< 0.05). The seropositive rate of RV-IgG in the young age group was higher than that in the elderly age group (80.16% vs 66.95%, P< 0.05), while the HSV Ⅰ/Ⅱ-IgG and CMVIgG positive rates in the young age group were lower than those in the elderly age group (83.23% vs 91.82%; 95.91% vs 96.99%, P< 0.05, respectively). Conclusion HSVⅠ/Ⅱ-IgM shows the highest seropositive rate in TORCH-IgM screen in Chengdu childbearing women, especially in the young age group, so the publicity of pre-pregnancy testing should be strengthened. In addition, the seropositive rate of CMV-IgM is the lowest, while the positive rate of CMV-IgG is the highest in this investigation. It may be due to the advocation of breastfeeding in China. CMV-IgG antibody has been produced in adults, and can be transferred to fetus from mother, so the primary infection is rare.