Abstract: Objective To explore the risk factors of catheter-related urinary tract infection (catheter-associated urinary tract infections, CAUTI) in critically ill patients, and provide evidences for the development of preventive measures. Methods One thousand and twenty-six critically ill patients in the Third People's Hospital of Haikou were selected and divided into CAUTI group(n=61) and non CAUTI group (n=965) according to whether they had CAUTI. Univariate analysis and multiple stepwise logistic regression were used to analyze the risk factors associated with CAUTI in severe patients. Results The incidence of CAUTI in 1 026 severe patients was 5.95% (61/1 026), and the rate of 1 000 day infection was 4.93% (61/12 379). A total of 71 strains of pathogens were isolated from 61 cases with CAUTI, including gram negtive bacteria (43.66%), fungi (30.99%) and gram positive bacteria (25.35%). Univariate and multivariate logistic regression analysis showed that the duration of mechanical ventilationOR, 3.142 (95% CI, 2.316-4.108), invasive operationOR, 1.629 (95% CI, 1.163-2.108), duration of catheterizationOR, 2.618 (95%CI, 1.985-3.228) and duration of comaOR, 1.705 (95% CI, 1.214-2.206) were independent risk factors of critically ill patients with catheter-associated urinary tract infections. Conclusion Duration of mechanical ventilation, invasive operation, duration of catheterization and duration of coma are independent risk factors of catheter-associated urinary tract infection in critically ill patients, and corresponding measures should be taken to reduce the incidence of CAUTI.