二次谐波成像技术在腱病动物实验研究中的应用综述

Application of second harmonic generation imaging in tendinopathy research

摘要: 二次谐波成像是利用非线性光学效应对组织进行成像的一种技术，具有无需染色、空间分辨率高、穿透力强、成像亮度和信噪比高、光漂白性小、无光毒性等优点，在生物医学研究领域已经得到广泛应用。肌腱组织主要成分为胶原纤维，其组织特性非常符合二次谐波成像介质的条件。本文简述了二次谐波成像的特点及其在当前腱病研究中的状况及遇到的困难，并简析了其在腱病研究中的应用前景。

Abstract: Second harmonic generation imaging (SHG-IM), as a novel nonlinear optical imaging technique, has been widely used in biomedical research. This label-free imaging technique has many advantages, such as higher spatial resolution, greater depth of penetration, higher singal-noise ratio, less photobleaching, nonphototoxicity and so on. The tendon tissues are rich in collagen fi bers, which exhibit endogenous SHG signals. Therefore, tendons can be well studied by SHG-IM technique. In this review, we have overviewed the characteristics of the SHG-IM technique, and discussed the difficulties and prospect of applications of SHG-IM in tendinopathy research.