B细胞非霍奇金淋巴瘤组织中YAP1和P53的表达及意义

Expression of YAP1 and P53 in B cell non-Hodgkin's lymphoma

  • 摘要: 目的 探讨YAP1和P53蛋白在B细胞非霍奇金淋巴瘤(B cell non-Hodgkin's lymphoma,B-NHL)组织中的表达意义及相互关系。 方法 应用组织芯片和免疫组织化学染色法检测广州医科大学附属第一医院2012年1月- 2017年12月经病理科确诊的60例B-NHL(病例组)与25例反应性淋巴组织增生(对照组)中YAP1和P53蛋白的表达,60例B-NHL包括弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL) 49例,小淋巴细胞淋巴瘤7例,套细胞淋巴瘤4例,后两者归为惰性组B-NHL。 结果 YAP1在B-NHL中的总体表达阳性率为68.3%,其中在DLBCL中的表达率为61.2%,惰性组B-NHL和对照组的阳性率均为100%,YAP1蛋白在DLBCL表达阳性率低于惰性组B-NHL和对照组(P均< 0.05)。P53在B-NHL中的总体表达阳性率为16.7%,其中在DLBCL和惰性组B-NHL的表达阳性率分别为18.4%和9.1%,在对照组中未见表达。P53在B-NHL和对照组中表达差异有统计学意义(P< 0.05)。YAP1和P53在B-NHL中的表达无明显相关性(P> 0.05)。 结论 YAP1可能作为肿瘤抑制因子参与了部分B-NHL的发生发展,但与P53蛋白表达无关。

     

    Abstract: Objective To investigate the expression, significance of YAP1 and P53 protein in B cell non-Hodgkin's lymphoma(B-NHL) and the relationship between these two indicators. Methods Tissue microarray and immunohistochemistry were used to detect the expression of YAP1 and P53 protein in 60 cases of B-NHL (case group) and 25 cases of reactive lymphoid hyperplasia(control group) which were pathologically confirmed in the First Affiliated Hospital of Guangzhou Medical University from January 2012 to December 2017. B-NHL were further categorized into DLBCL group (49 cases) and indolent B-NHL group (7 cases of SLL and 4 cases of MCL). Results The overall positive rate of YAP1 in B-NHL was 68.3%, and it was 61.2% in diffuse large B cell lymphoma (DLBCL), 100% in indolent B-NHL group and control group, respectively. The positive rate of YAP1 protein in DLBCL was lower than that in the indolent B-NHL group and control group, and the difference was statistically significant (all P< 0.05).The overall positive rate of P53 in B-NHL was 16.7%, and 18.4% in diffuse large B cell lymphoma (DLBCL), 9.1% in indolent group, negative in control group, respectively. There was significant difference in the expression of P53 protein in B-NHL and control group (P< 0.05). There was no significant correlation between YAP1 and P53 expression in B-NHL (P> 0.05). Conclusion YAP1 may be involved in the occurrence and development of some B-NHL as tumor suppressor genes, and its expression is unrelated to P53 overexpression.

     

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