Abstract: Objective To investigate the relationship between different prenatal diagnostic indications and fetal abnormal karyotypes. Methods The amniotic fluids or cord bloods were collected in 3 226 pregnant women with prenatal diagnosis indications in our center from 2014 to 2018.The chromosomal karyotypes and the abnormal chromosomal karyotype detection rate were analyzed. Results A total of 289 fetal chromosomal abnormalities(excluding chromosomal polymorphisms)were detected in 3 226 karyotypes,with detection rate of 8.96%.The abnormal karyotype detection rates in single indication group,two indications group and three indications group were 6.81%,38.57%,and 75.00% respectively,with statistically significant differences between these groups(P<0.05,respectively).The abnormal karyotype detection rate in NIPT-positive subgroup(56.38%)was the highest in the single indication group,followed by the parental chromosomal abnormality subgroup(52.78%). Conclusion The abnormal chromosomal karyotype detection rate of NIPT-positive group and parental chromosomal abnormalities group ranks highest in all the indications.Pregnant women with more prenatal diagnosis indications have higher risk of fetal chromosomal abnormalities.