Abstract: Objective To observe the effect of hydrogen-rich water,traditional Chinese medicine(Jinfukang oral liquid,JFK)or their combined intervention on tumorigenesis of cholangiocarcinoma. Methods Adult male SD rats were divided into four groups in this study,including the control group(n=10),the hydrogen-rich water group(n=10),the JFK group(n=5)and the hydrogen-rich water+JFK group(n=5).Rats in control group were given free-drinking water containing thioacetamide(300 mg/L),while those in the experimental groups were given drinking water containing thioacetamide as well as hydrogen-rich water,JFK,or both.At 20 weeks after initiation,all rats were sacrificed,and the livers were carefully examined under the microscope. Results All ratable rats(n=9)(9/9)in the control group formed visible or microscopic tumors at 20 weeks,57.1%of rats(4/7)developed microscopic tumors in the hydrogen-rich water group,40%(2/5)developed microscopic tumors in the JFK group,while none of the rats(0/5)developed tumors in the hydrogen-rich water+JFK group. Conclusion The synergistic effect of hydrogen-enriched water and JFK can successfully block the occurrence of intrahepatic cholangiocarcinoma in rats.