Abstract: Objective To compare the disease uncertainty,anxiety and depression scale in patients with glaucoma versus patients with cataract,and explore their related influencing factors. Methods One hundred and sixty patients (average age of 59.20 years) with primary glaucoma and 150 patients (average age of 60.32 years) with cataracts were recruited sequentially in Beijing Fengtai Hospital from January 2014 to June 2018.All the participants completed the questionnaires for socio-demographic information,Mishel uncertainty in illness scale (MUIS) and hospital anxiety and depression scale (HADS). Results The MUIS and HADS scores of glaucoma patients were significantly higher than those of cataract patients (MUIS,90.37±12.66 vs 83.29±11.25;HADS,12.16±7.81 vs 8.43±5.29;P<0.05,respectively).The results of multivariate logistic regression analysis showed that the disease uncertainty of glaucoma patients was significantly correlated with HADS score (OR=1.37,95% CI,1.31 to 1.43).Anxiety was significantly correlated with the visual acuity (VA) of healthy lateral eyes (OR=0.36,95% CI,0.15 to 0.82) and MUIS score (OR=1.07,95% CI,1.01-1.13).Depression was significantly correlated with educational level (OR=0.54,95% CI,0.27 to 0.86).The sense of uncertainty in illness in cataract patients was significantly correlated with HADS-a score (OR=1.28,95% CI,1.13 to 1.46).Anxiety was significantly correlated with the VA of the healthy eye (OR=0.33,95% CI,0.18 to 0.69) and VA of the eye with cataract (OR=1.22,95% CI,1.06 to 1.44),Depression was significantly correlated with MUIS score (OR=3.02,95% CI,1.00 to 9.12). Conclusion The incidence of uncertainty in illness,anxiety and depression in glaucoma patients is higher than that in cataract patients.