Abstract: Objective To identify the differentially expressed of genes in adipose stromal cells in healthy people versus patients with multiple myeloma （MM）,and provide evidence for MM studies. Methods The GSE133346 microarray data chip related to multiple myeloma and adipose stromal cells were downloaded from the Gene Expression Omnibus GEO.The data was standardized,calibrated,Log₂ transformed,and the gene ID and supplemental deletion were converted by R language software.Then | log fold change （FC） |>1.5 and P<0.05 were used to screen the calibrated expressed genes in the database.Differentially expressed genes were inputted into the DAVID online database to obtain GO enrichment analysis results,including biological processes （BP）,cellular components （CC） and molecular functions （MF）.At the same time,the KEGG pathway enrichment analysis results were also obtained from the DAVID online database.The STRING online database was used to analyze the selected differentially expressed genes,and Cytoscape was used to perform the topological analysis to identify the key differentially expressed genes.The critical prognostic genes were evaluated by UALCAN of cancer data to assess the survival by differentially expressed genes. Results A total of 148 differentially expressed genes were screened for adipose stromal cells in 12 healthy controls and 12 MM patients,of which 47 were up-regulated and 101 were down-regulated.GO and KEGG enrichment analysis results showed that BP was mainly concentrated in leukocyte migration,cell adhesion,cell response to fibroblast growth factor stimulation,and extracellular matrix tissue;CC was mainly concentrated in extracellular exosomes,endoplasmic reticulum cavity,extracellular area,cell surface and plasma membrane;while MF was mainly concentrated in collagen binding,actin binding,platelet-derived growth factor receptor binding,extracellular matrix structural components,and tubulin binding.On the KEGG pathway,differentially expressed genes were enriched in adhesion,regulating actin cytoskeleton,ECM-receptor interactions,choline metabolism in cancer,and the PI3K-Akt signaling pathway.Topological analysis showed that there were 30 key differentially expressed genes,14 were up-regulated and 16 were down-regulated.UALCAN analysis showed that 6 differentially expressed genes were related to the survival of MM patients,including CSRP1,FYN,MYLK,FSTL3,LAMB1,PRRX1.Among them,CSRP1,FYN and MYLK were positively correlated with MM progressing,while FSTL3,LAMB1,PRRX1 were negatively correlated with MM progressing. Conclusion In the analysis of the microarray data chip of adipose stromal cells in multiple myeloma or healthy people,six key differentially expressed genes are identified to be related to pognosis of MM.