Abstract: Objective To investigate the effects of triptolide combined with cisplatin on the growth,apoptosis and invasion of Hela/DDP cell lines of cervical cancer and its mechanism. Methods Hela/DDP cells were randomly divided into control group,platinum group (100 mg/ml),triptolide group (80 nmol/L) and combined group (100 mg/ml DDP and 80 nmol/l triptolide).The growth rate of each group was detected by CCK-8 assay,the apoptosis rate of each group was detected by flow cytometry,the cell invasion ability was detected by Transwell chamber technology,and expression of apoptosis-related protein Bcl-2,FLIP,XIAP was detected by Western blot. Results Triptolide could inhibit the proliferation of cisplatin resistance cell line Hela/DDP,promote cell apoptosis and reduce the ability of cell invasion (The cell inhibition rates of blank control group,cisplatin group,triptolide group and combined group were 0,21.68±3.40%,32.60±5.21%,58.11±3.59%,the apoptosis rates were 2.87±0.63%,15.16±1.13%,21.66±1.46%,33.35±0.93%,and the number of cells passing the Transwell chamber were 304.33±17.21,271.67±7.51,261.67±5.69,196.00±14.93).Compared to DDP treatment,combination of DDP and TP had a significant inhibition effect (P<0.05).At the molecular level,the expression of Bcl-2 protein in blank control group,cisplatin group,Triptolide group and combined group were 1.05±0.08,0.86±0.05,0.75±0.13,0.57±0.11,the expression of XIAP were 1.05±0.06,0.68±0.01,0.67±0.03,0.38±0.02,the expression of FLIP were 0.72±0.27,0.54±0.02,0.41±0.07,0.35±0.03.Compared to blank control group,the expression of Bcl-2 and XIAP protein decreased significantly in cisplatin group,triptolide group and combined group (all P<0.05),and the expression of FLIP protein in Triptolide group and combination group also decreased significantly (all P<0.05). Conclusion The combination of triptolide and cisplatin can inhibit the growth of Hela/ DDP cells and induce cell apoptosis and reduce the resistance of Hela/DDP cells to cisplatin,and its molecular mechanism may be related to the down-regulation of the expression of Bcl-2,FLIP,XIAP protein.