Abstract: Objective To observe the clinical effects of auricular point sticking combined with diaphragmatic breathing exercise on stress-induced sleep disorder following acute high-altitude exposure. Methods In September 2019,236 subjects with ISI scale score greater than 7 were randomly divided into auricular point group (n=66),breathing group (n=72),combined group (n=45) and control group (n=53).The subjects were all male with an average age of (18.95±1.07) years (range,18-24 years).The auricular point group was given auricular point sticking.The breathing group received training of diaphragm-breathing exercise.The combined group received auricular point sticking and diaphragm-breathing training.The control group recieved no therapies.ISI scale,Fatigue Self-assessment scale and Profile of Mood States (Vigor score) were used as evaluation indexes. Results Totally 208 cases completed the program (58 cases in the auricular point group,66 cases in the breathing group,36 cases in the combined group and 48 cases in the control group).Compared with the control group,the ISI scores of the auricular point group and the combined group on the second day were significantly lower (8.21±0.77 vs 7.53±0.82 and 7.22±0.68).And the ISI score of the auricular point group,the breathing group and the combined group on the sixth day of treatment were also significantly lower than that of the control group (6.95±0.51,7.32±0.59,6.44±0.61 vs 7.83±0.56) (all P<0.05).The score of Fatigue Self-assessment scale on the first day of treatment in the auricular point group and the combined group was significantly lower than that of the control group (8.55±0.84 and 8.19±1.19 vs 9.81±1.10,P<0.05,respectively).And the scores of the auricular point group,the breathing group and the combined group on the second and sixth day of treatment reduced significantly when compared with control group (2 d:7.26±0.83,7.55±1.13,7.17±1.13 vs 8.73±1.07;6 d:6.22±0.92,6.42±1.15,5.78±1.22 vs 7.73±1.35;all P<0.05).Compared with the control group,the Vigor score of the breathing group and the combined group on the first and second day of treatment increased significantly (1 d:12.62±1.83,12.83±1.42 vs 11.58±1.47;2 d:13.97±1.97,14.67±1.91 vs 11.73±1.72;all P<0.05).And it was also higher in the auricular point group,the breathing group and the combined group when compared with control group on the sixth day of treatment (12.50±1.54,14.45±1.87,15.53±1.86 vs 11.81±1.55,P<0.05.respectively).The combined group was superior to the breathing group and the auricular group in terms of all the indicators mentioned above on the sixth day (all P<0.05). Conclusion Bothauricular point sticking and diaphragm breathing can improve sleep quality,reduce fatigue and increase vigor for cases with high altitude exposure,and the combination will work better.