Abstract: Objective To explore the value of alpha-fetoprotein (AFP),beta-human chorionic gonadotropin (β-hCG) and uncon-jugated estriol (uE3) in serum during the second trimester combined with peak systolic velocity/end diastolic velocity (S/D) detected by doppler ultrasound in the third trimester in predicting fetal growth restriction (FGR). Methods The mid-pregnancy Down's screening indicators (expressed as multiples of the medianMoM),doppler ultrasound indexes in the third trimester of pregnant women who gave birth in our hospital from January 1,2018 to December 31,2018 were collected,then they were divided into fetal growth restriction (FGR) group (n=85) and normal control group (n=809) according to the pregnancy outcomes.The receiver operating curve was applied to evaluate the performance of each index in predicting FGR individually and jointly. Results There was no significant difference in BMI and gender between the FGR group and the normal pregnancy group (P>0.05).The values of AFP,β-hCG MoM and S/D in FGR group were significantly higher than those in the control group (all P<0.05),while the value of uE3 mom in FGR group was significantly lower than that in the control group (P<0.05).The AUC of FGR predicted by AFP,β-hCG,uE3 and S/D was 0.836,0.724,0.786 and 0.741,respectively,while the AUC of FGR predicted by four indexes combination was 0.937,which was higher than that predicted by each single index (P<0.05,respectively). Conclusion The combination of AFP,β-hCG,uE3 and S/D value is superior to each factor alone for predicting FGR,and is of great value in clinical diagnosis.