细胞因子Irisin缓解高糖诱导骨髓间充质干细胞凋亡的机制研究

郭树琴; 焦婷; 赵立升; 杨烁; 党瑞杰; 温宁; 朱彪

doi:10.3969/j.issn.2095-5227.2021.06.008

细胞因子Irisin缓解高糖诱导骨髓间充质干细胞凋亡的机制研究

Mechanisms of irisin attenuates cell apoptosis of bone-marrow mesenchymal stem cells in high glucose condition

摘要

摘要:
背景高糖可诱导骨髓间充质干细胞(bone-marrow mesenchymal stem cells，BMSCs)凋亡，而细胞因子Irisin对糖毒性所致的细胞损伤具有保护作用。
目的探讨细胞因子Irisin对高糖环境中BMSCs凋亡的影响及其作用机制。
方法复苏BMSCs并将其分为对照组(Vehicle)、高糖诱导组(high glucose，HG)、Irisin干预组(HG + irisin；Irisin)和抑制剂组(HG + irisin + compound C，CC)。各组细胞培养3 d后，流式细胞术检测细胞凋亡率。Western blotting检测凋亡相关蛋白Bcl-2/Bax比值和磷酸化AMP依赖的蛋白激酶(phosphorylated AMP-activated protein kinase，p-AMPK)、AMPK的表达水平。
结果与Vehicle组相比，HG组BMSCs细胞凋亡率显著升高(P＜0.05)；Irisin可降低高糖诱导的BMSCs细胞凋亡(P＜0.05)，提高Bcl-2/Bax的蛋白表达比(P＜0.05)，同时激活AMPK。当特异性地抑制AMPK后，Irisin缓解高糖诱导BMSCs凋亡的作用明显减弱。
结论 Irisin可通过激活AMPK缓解高糖诱导的BMSCs凋亡。

Abstract:
Background Previous studies revealed that high glucose could induce bone-marrow mesenchymal stem cells apoptosis, and irisin attenuated cell injury resulted from glucotoxicity.
Objective To investigate the effects of irisin on cell apoptosis of bone-marrow mesenchymal stem cells (BMSCs) in high glucose condition.
Methods BMSCs were divided into Vehicle group, HG (high glucose) group, Irisin (HG plus irisin intervention) group, and CC (HG + irisin + Compound C (CC) group. After cultured for 3 days, flow cytometry was used to evaluate apoptotic rate, and Western blotting was used to investigate protein level of Bcl-2/Bax and phosphorylated AMP-activated protein kinase (p-AMPK).
Results Compared with the Vehicle group, apoptotic rate of HG group increased obviously (P＜0.05); while Irisin intervention could reduce apoptotic rate (P＜0.05), increase Bcl-2/Bax expression (P＜0.05), and activate AMPK. However, when inhibiting AMPK, the effects of Irisin on inhibiting cell apoptosis were blunted (P＜0.05).
Conclusion Irisin can inhibit cell apoptosis of BMSCs in high glucose condition via activating AMPK.

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