Abstract: Proteomics studies protein composition and its changes in cells, tissues and organisms. Proteomics technology, as an important tool, can solve many problems in medicine and biology. Nowadays, a variety of proteomic analysis methods have been widely applied in the research of respiratory diseases, these methods provide a new way in the research of the pathogenesis and targeted drugs. This paper summarizes the research progress of proteomics and discusses its application in the study of respiratory diseases.