Abstract: Objective To provide the reference for the diagnosis and treatment of catheter-related bloodstream infection（CRBSI） by analyzing the distribution of pathogens in CRBSI. Methods One hundred and eighty patients with positive central vein culture of CRBSI admitted to our hospital from September 2008 to December 2010 were retrospectively analyzed. Results Of the 180 patients,56（31.1%） were diagnosed as CRBSI,including 29 in Intensive Care Unit,8 in Tuberculosis Department,6 in Department of Kidney Medicine,4 in Department of Respiratory Medicine,8 in other departments.Of the 56 pathogens,24（42.8%） were Gram-negative bacilli（mainly Acinetobacter baumannii and Klebsiella pneumoniae）,22（39.3%） were Gram–positive cocci（mainly coagulase-negative staphylococci and S.aureus）,and 10（17.9%） were Candida. Conclusion Great importance should be attached to the multi-drug resistant Acinetobacter baumannii and fungi which are present in CRBSI.