Abstract: Objective To provide the evidence for rational use of antibiotics in clinical practice by investigating the distribution of blood cultured bacteria in 2008-2010 and changes in their drug resistance. Methods Bacteria isolated from blood samples were cultured with BACTEK 9050 and identified with Microscan walkaway-96 and then underwent drug sensitivity test. Results The rate of isolated coagulase negative staphylococci（CNS） was the highest followed by Escherichia coli（E.coli）.Klebsiella pneumoniae（K.pneumoniae）,staphylococcus aureus（S.aureus）,Bowwan/hemolytic amotile bacteria,and Pseudomonas aeruginosa were the main pathogens leading to blood infection.The sensitivity of E.coli and K.pneumoniae to imipenem was 100% and rather high to amikacin and pipracillin-tazobatan.The sensitivity of Bowwan/hemolytic amotile bacteria and Pseudomonas aeruginosa to different antibiotics was also rather high.No S.aureus,CNS and enterococci were found to be sensitive to vancomycin and linezolid. Conclusion Different bacteria can be isolated from blood cultured samples and have a rather high drug resistance.More attention should be paid to the drug resistance of blood infection bacteria both in clinical practice and in laboratory.