Abstract: Objective To compare the two detection methods of metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii. Methods Metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii was detected by modified Hodge test and Etest MBL test.Resistance of Acinetobacter Baumanni to imipenem was assayed with McrioScan WalkAway 96. Results The metallo-β-lactamase was detected in 40 imipenem-resistant Acinetobacter Baumannii strains with the two methods.The metallo-β-lactamase was detected in 36 out of the 40 imipenem-resistant Acinetobacter Baumannii strains by modified Hodge test and in 38 imipenem-resistant Acinetobacter Baumannii strains by Etest MBL test,with a positive detection rate of 90% and 95%,respectively. Conclusion The two detection methods are simple,rapid and accurate for detecting the metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.