Abstract: Objective To study the application of CY211 in diagnosis of early metastasis and differentiation of pancreatic cancer. Methods Serum was collected from patients with pancreatic cancer.Patients were divided into two groups based on positive and negative lymph nodes and their differentiation level.Their CY211,CA199,lipase（LPS） and amylase（AMY） levels were measured and compared with those in controls. Results The ROC area of pancreatic cancer was 96.7% when combined detection of CY211,CA199 and LPS was used in its diagnosis.The sensitivity of combined detection of CY211,CA199 and LPS was 92%.The serum CY211 level was higher in lymph node positive group and poor differentiation group than in lymph node negative group and high differentiation group（P＜0.01）. Conclusion CY211 can help the diagnosis of pancreatic cancer and combined detection of CY211,CA199 and LPS can increase the sensitivity of pancreatic cancer diagnosis and plays a role in identification of metastasis and differentiation of pancreatic cancer.